Composite
Part:BBa_J64708:Design
Designed by: laura lavery Group: Voigt Lab (2007-03-19)
Trp Operon (B. Subtilis)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 6570
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3106
Illegal PstI site found at 6570 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3177
Illegal XhoI site found at 1889 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 6570
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 6570
Illegal NgoMIV site found at 3968
Illegal NgoMIV site found at 4496
Illegal NgoMIV site found at 4956
Illegal NgoMIV site found at 5657
Illegal AgeI site found at 230
Illegal AgeI site found at 4950
Illegal AgeI site found at 6348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5526
Illegal BsaI.rc site found at 692
Illegal SapI.rc site found at 839
Illegal SapI.rc site found at 2327
Illegal SapI.rc site found at 3254
Design Notes
none
Source
(Henner DJ. et. al., Gene, 1984)